foil for nucleic acid analysis based on isothermal recombina

ONE-STEP DIGITAL PLASMA SEPARATION FOR .

ONE-STEP DIGITAL PLASMA SEPARATION FOR MOLECULAR DIAGNOSTICS Erh-Chia Yeh1* and Luke P. Lee1,2 1Departments of Bioengineering, and BSAC, University of California, Berkeley, USA 2Departments of EECS, and Biophysics, University of California, Berkeley, USA ABSTRACT Current blood-based quantitative nucleic acid (NA) detection requires many .

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Current and Future Perspectives on Isothermal Nucleic Acid ...

Oct 19, 2019· Lutz S, Weber P, Focke M, et al. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip. 2010;10(7):887–893. doi:10.1039/b921140c. 112. Euler M, Wang Y, Otto P, et al. Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.

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[PDF] LabDisk with complete reagent prestorage for sample ...

Portable point-of-care devices for pathogen detection require easy, minimal and user-friendly handling steps and need to have the same diagnostic performance compared to centralized laboratories. In this work we present a fully automated sample-to-answer detection of influenza A H3N2 virus in a centrifugal LabDisk with complete prestorage of reagents. Thus, the initial supply of the sample ...

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ONE-STEP DIGITAL PLASMA SEPARATION FOR .

ONE-STEP DIGITAL PLASMA SEPARATION FOR MOLECULAR DIAGNOSTICS Erh-Chia Yeh1* and Luke P. Lee1,2 1Departments of Bioengineering, and BSAC, University of California, Berkeley, USA 2Departments of EECS, and Biophysics, University of California, Berkeley, USA ABSTRACT Current blood-based quantitative nucleic acid (NA) detection requires many sample preparation steps.

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SARS-CoV-2 detection using isothermal amplification and a ...

Sep 29, 2020· This work describes the optimization of a sample preparation and detection pipeline for a SARS-CoV-2 diagnostic test that is rapid and does not require specialized equipment. This pipeline consists of viral inactivation, rendering samples safer to work with, followed by a sensitive 30-min isothermal detection reaction with a color-based red to yellow .

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Parallel solid-phase isothermal amplification and ...

This research has been funded through projects FEDER PrometeoII/2014/040 (GVA), and CTQ/2013/45875-R (MINECO). The Spanish Ministry of Education and Science provided S.S.F. with a grant for PhD studies.

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Nucleic acid diagnostics on the total integrated lab-on .

9/15/2019· Cell lysis and nucleic acid purification is an initial step and one of the most vital steps for molecular diagnostics. Typical cell lysis methods applied in the diagnostic chips are based on thermal, chemical, electrochemical, and mechanical ones. The most popular type of nucleic acid purification on the LOAD is solid-phase extraction.

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Isothermal DNA amplification in bioanalysis: strategies ...

Jan 20, 2011· 78 Lutz S, Weber P, Focke M et al. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10(7),887–893 ( 2010 ). Crossref, Medline, CAS, Google Scholar

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Genie II - OptiGene

LAMP Isothermal Amplification Using LAMP, a novel isothermal nucleic acid amplification technology developed by Eiken Chemical Co., Japan, together with the Genie® II instrument, a rapid test was produced for the detection of Shiga toxin-producing E. coli O104 (STEC O104:H4). From the published genome sequence, LAMP primers were designed

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[PDF] Multiplex isothermal solid-phase recombinase ...

AbstractWe report on the development of an on-chip RPA (recombinase polymerase amplification) with simultaneous multiplex isothermal amplification and detection on a solid surface. The isothermal RPA was applied to amplify specific target sequences from the pathogens Neisseria gonorrhoeae, Salmonella enterica and methicillin-resistant Staphylococcus aureus (MRSA) using genomic DNA.

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Recombinase Polymerase Amplification: A New DNA/RNA ...

Cite this article: GAO Wei-Fang,ZHU Peng,HUANG Hai-Long. Recombinase Polymerase Amplification: A New DNA/RNA Amplification Strategy[J]. Chinese Journal of .

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Isothermal Recombinase Polymerase Amplification Assay ...

Apr 01, 2014· We demonstrate the potential of isothermal recombinase polymerase amplification assay as a clinically useful molecular diagnostic tool that is simple and faster than PCR/RT-PCR. Recombinase polymerase amplification offers great potential for nucleic acid–based .

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US20080182312A1 - Stable reagents and kits useful in loop ...

Provided herein is a reagent preparation for loop-mediated isothermal amplification of nucleic acids comprising: at least one polymerase enzyme, a target-specific primer set, and dinucleotide triphosphates (dNTPs) in a single, dry format; wherein said reagent preparation is water soluble and stable above 4° C.

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Rapid Detection of Shrimp White Spot Syndrome Virus by ...

Virology 266 : 227 - 236 . 39. Lutz S, Weber P, Focke M, Faltin B, Hoffmann J, et al. ( 2010 ) Microfluidic labon-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA) . Lab on a Chip 10 : 887 - 893 . 40 . Q ia ge n w e b si te .

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Development of a Panel of Recombinase Polymerase ...

Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents. The panel included RPAs for Francisella tularensis, Yersinia pestis, Bacillus anthracis, variola virus ...

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Current and Future Perspectives on Isothermal Nucleic .

10/19/2019· Lutz S, Weber P, Focke M, et al. Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip. 2010;10(7):887–893. doi:10.1039/b921140c. 112. Euler M, Wang Y, Otto P, et al. Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.

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Recombinase polymerase amplification(RPA) is a kind of isothermal nucleic acid amplification technique rising in recent years. RPA is more rapid, convenient and efficient than the polymerase chain reaction (PCR) and other isothermal amplification techniques. In this paper, this new technique will be introduced in detail, with reviewing over the ...

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Ultrasensitive CRISPR-based diagnostic for field ...

Oct 13, 2020· Detection of submicroscopic malaria in asymptomatic individuals is needed for eradication and remains a diagnostic gap in resource-limited settings. Nonfalciparum clinical diagnostics are a second gap, as these infections have a low parasite density and are commonly undetected. We describe an integrated, 60-min, ultrasensitive and specific CRISPR-based diagnostic for the four major .

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Microfluidic Devices for Nucleic Acid (NA) Isolation ...

Lutz S, Weber P, Focke M, Faltin B, Hoffman J, Müller C, Mark D, Roth G, Munday P, Armes N, Piepenberg O, Zengerle R, von Stetten F (2010) Microfluidic lab-on-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab .

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Sequence dependence of isothermal DNA amplification via ...

https://academic.oup/nar/article/40/11/e87/2409857Introduction

Nucleic Acid Detection and Analysis—Chapter 8 | Thermo ...

Chapter 8—Nucleic Acid Detection and Analysis Nucleic Acid Stains—Section 8.1; Labeling Oligonucleotides and Nucleic Acids—Section 8.2; Nucleic Acid Quantitation in Solution—Section 8.3; Nucleic Acid Detection on Gels, Blots and Arrays—Section 8.4

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Recent developments in nucleic acid identification using ...

2/4/2014· Recent developments in nucleic acid identification using solid-phase enzymatic assays Recent developments in nucleic acid identification using solid-phase enzymatic assays Khodakov, Dmitriy; Ellis, Amanda 2014-02-04 00:00:00 This review (containing 101 refs.) covers recent achievements in the development of new approaches for enzymatically assisted detection of nucleic .

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Web COBIOPHAD MAY 19

For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists

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Microfluidics: innovative approaches for rapid diagnosis ...

For on-site analysis, sample pre-processing is often required in order to obtain nucleic acids that are suitable for microfluidic analysis . Various methods such as exposure to heat, lytic enzymes and different kinds of chemicals have been utilized to facilitate cell disruption in order to extract the DNA from the specimen [ 38, 40, 42, 43 ].

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Point-of-care nucleic acid testing for infectious diseases ...

5/1/2011· Isothermal nucleic acid amplification methods that are based on polymerase extension plus a single-strand cutting event. In SDA (Becton Dickinson) 42, 43, NEMA (Ustar) and ICA (RapleGene), an intermediate target is generated using strand-displacing amplification via sacial outer bumper primers.

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US20080182312A1 - Stable reagents and kits useful in loop ...

Provided herein is a reagent preparation for loop-mediated isothermal amplification of nucleic acids comprising: at least one polymerase enzyme, a target-specific primer set, and dinucleotide triphosphates (dNTPs) in a single, dry format; wherein said reagent preparation is water soluble and stable above 4° C.

Get price

Microfluidic-Based Nucleic Acid Amplification Systems in ...

Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples.

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[PDF] LabDisk with complete reagent prestorage for sample ...

Portable point-of-care devices for pathogen detection require easy, minimal and user-friendly handling steps and need to have the same diagnostic performance compared to centralized laboratories. In this work we present a fully automated sample-to-answer detection of influenza A H3N2 virus in a centrifugal LabDisk with complete prestorage of reagents. Thus, the initial supply of the sample ...

Get price

Isothermal Amplification | NEB

Loop-mediated isothermal amplification for detection of nucleic acids Curr Protoc Mol Biol. 105, PubMedID: 24510439, DOI: Legal Information This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

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A Convenient Nucleic Acid Test on the Basis of the ...

May 21, 2014· The recent and continuing epidemic of enterovirus 71 in China has affected millions of children and resulted in thousands of deaths. Timely diagnosis and management is essential for disease control. Current enterovirus 71 molecular tests require resources that are unavailable for on-site testing. We have developed a simple-to-operate nucleic acid test, the convenient and integrated nucleic ...

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Purification and some of the functions of the products of ...

Sascha Lutz, Patrick Weber, Max Focke, Bernd Faltin, Jochen Hoffmann, Claas Müller, Daniel Mark, Günter Roth, Peter Munday, Niall Armes, Olaf Piepenburg, Roland Zengerle, Felix von Stetten, Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA), Lab on a Chip, 10.1039/b921140c, 10 ...

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